ABSTRACT
Objective:To explore the leaves of Hydrocotylejavanica Thunb.as a source of safe and effective antibacterial herbal medicine.Methods:The standardization was validated by stepwise physicochemical studies,element analysis,determination of ash values,fluorescence analysis,assessment of moisture content,extractive values in different solvent systems and extraction methods.Heavy metal contents,mineral and element contents were analysed by atomic absorption spectrophotometry,inductively coupled plasma-mass spectrometer and CHNS/O analyser,respectively.Results:The methanol extract of the folklore medicinal plant having antibacterial efficacy contained flavonoids and phenolic OH groups.The ICP multi standard indicated the presence of three major compounds with molecular mass of 161 190 and 221 Da.Heavy metals viz.lead,mercury and copper content were 4.38 ppm,＜ 0.05 ppm and 24.70 ppm,respectively.Minerals content of calcium,phosphorus,potassium and iron were 1 190.94 mg/100 g,375.57 mg/100 g,2820 mg/100 g and 340.20 mg/100 g of plant sample,respectively.Elements like carbon,hydrogen,nitrogen and sulphur contents were 38.18％,5.67％,2.23％ and 0.51％,respectively.Heavy metal profile of the tested plant was within the permissible limits of the regulatory authorities.Conclusions:Hence the present physicochemical and elements studies reveals that the plant Hydrocotylejavanica Thunb.could be a potent source of herbal preparation as well as a safe and novel synthetic antibacterial drug.
ABSTRACT
Natural bioactive compounds from plants are of great importance in modem therapeutics,which are used to prepare antibiotics,growth supplements or some other therapeutics.L-theanine is such a bioactive amide amino acid presented in different plants and fungi,especially in tea.Theanine has influential effects on lifestyle associated diseases,such as diabetes,cardiovascular disorders,hypertension,stress relief,tumor suppression,menstruation and liver injury.This amino acid can maintain normal sleep and improve memory function and nullify effect of the neurotoxins.The rate of bioavailability and its medium of ingestion in the body is one of the great concerns for its additional antioxidant properties.Pharmacokinetics of the bioactive compound and its mode of action are described herewith.The biosynthesis and industrial synthesis are also reviewed to promote accelerated production of this bioactive compound in the pharmaceutical industries.